Ion channels have gained increased interest as therapeutic targets over recent years since a growing number of human and animal diseases have been attributed to defects in ion channel function. In the planar bilayer section methods are described for the expression of ion channels in xenopus laevis oocytes the isolation of their membranes the insertion of ion channels into the bilayer by vesicle fusion and the recording of single ion channel current measurements at a constant applied voltage the reconstitution of bacterial . The aim of ion channel reconstitution is to incorporate the channel into an artificial membrane in which its function can be investigated for practical purposes this means a membrane system in which ion flow through the channel can be studied under voltage clamp conditions ideally with good enough resolution to permit the. Scanty understanding of channel structures and because of the difficulty in iso lating functionally active channel proteins in this volume which can be considered a biochemically slanted companion to sakmann and nehers single channel recording i have tried to present a view of the current landscape of ion channel reconstitution. The reconstitution of ion channel proteins in artificially formed blms represents a well defined system for the functional analysis of ion channels and screening the effects of drugs that act on them
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